NSF ELISA Kit

Catalog No. ABIN2044029

Product Details NSF ELISA Kit

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Minimum Detection Limit: 0.31 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.116 ng/mL

Application Details

Plate: Pre-coated

Protocol: N-Ethylmaleimide Sensitive Factor (NSF) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Ethylmaleimide Sensitive Factor (NSF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Ethylmaleimide Sensitive Factor (NSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Ethylmaleimide Sensitive Factor (NSF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of N-Ethylmaleimide Sensitive Factor (NSF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for NSF

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Alternative Name: N-Ethylmaleimide Sensitive Factor (NSF) (NSF Products)

Synonyms: SKD2 ELISA Kit, AI316878 ELISA Kit, AU020090 ELISA Kit, AU067812 ELISA Kit, NSF ELISA Kit, 18.m06598 ELISA Kit, nsf ELISA Kit, si:bz18k17.1 ELISA Kit, wu:fj33g11 ELISA Kit, N-ethylmaleimide sensitive factor ELISA Kit, T1J1.4 ELISA Kit, T1J1_4 ELISA Kit, NEM-sensitive fusion protein ELISA Kit, N-ethylmaleimide sensitive factor, vesicle fusing ATPase ELISA Kit, N-ethylmaleimide sensitive fusion protein ELISA Kit, N-ethylmaleimide-sensitive factor ELISA Kit, N-ethylmaleimide sensitive factor ELISA Kit, N-ethylmaleimide-sensitive fusion protein, putative ELISA Kit, N-ethylmaleimide sensitive factor L homeolog ELISA Kit, vesicle-fusing ATPase ELISA Kit, N-ethylmaleimide-sensitive factor a ELISA Kit, AAA-type ATPase family protein ELISA Kit, NSF ELISA Kit, Nsf ELISA Kit, nsf ELISA Kit, BBOV_II007200 ELISA Kit, TGME49_318510 ELISA Kit, nsf.L ELISA Kit, LOC100180685 ELISA Kit, LOC9310886 ELISA Kit, nsfa ELISA Kit