NEIL1 ELISA Kit

Catalog No. ABIN2044051

Human NEIL1 ELISA Kit Colorimetric assay for quantification of Human NEIL1.

Quick Overview for NEIL1 ELISA Kit (ABIN2044051)

Target: NEIL1 (Endonuclease VIII-Like 1 (NEIL1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details NEIL1 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.062 ng/mL

Application Details

Plate: Pre-coated

Protocol: Nei Endonuclease VIII Like Protein 1 (NEIL1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nei Endonuclease VIII Like Protein 1 (NEIL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nei Endonuclease VIII Like Protein 1 (NEIL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nei Endonuclease VIII Like Protein 1 (NEIL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Nei Endonuclease VIII Like Protein 1 (NEIL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for NEIL1

Target: NEIL1 (Endonuclease VIII-Like 1 (NEIL1))

Alternative Name: Nei Endonuclease VIII Like Protein 1 (NEIL1)

Pathways: DNA Damage Repair