NFASC ELISA Kit

Catalog No. ABIN2044073

Product Details NFASC ELISA Kit

Target: NFASC (Neurofascin (NFASC))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Minimum Detection Limit: 0.31 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.083 ng/mL

Application Details

Plate: Pre-coated

Protocol: Neurofascin (NFASC) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurofascin (NFASC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neurofascin (NFASC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neurofascin (NFASC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Neurofascin (NFASC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for NFASC

Target: NFASC (Neurofascin (NFASC))

Abstract: NFASC Products

Synonyms: sc:d0205 ELISA Kit, NF ELISA Kit, NRCAML ELISA Kit, AA387016 ELISA Kit, D430023G06Rik ELISA Kit, mKIAA0756 ELISA Kit, neurofascin ELISA Kit, neurofascin homolog (chicken) a ELISA Kit, NFASC ELISA Kit, nfasca ELISA Kit, Nfasc ELISA Kit

Pathways: Cell-Cell Junction Organization