NTSR1 ELISA Kit

Catalog No. ABIN2044085

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse NTSR1.

Quick Overview for NTSR1 ELISA Kit (ABIN2044085)

Target: NTSR1 (Neurotensin Receptor 1 (High Affinity) (NTSR1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details NTSR1 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.066 ng/mL

Application Details

Plate: Pre-coated

Protocol: Neurotensin Receptor 1, High Affinity (NTSR1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurotensin Receptor 1, High Affinity (NTSR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neurotensin Receptor 1, High Affinity (NTSR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neurotensin Receptor 1, High Affinity (NTSR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Neurotensin Receptor 1, High Affinity (NTSR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for NTSR1

Target: NTSR1 (Neurotensin Receptor 1 (High Affinity) (NTSR1))

Alternative Name: Neurotensin Receptor 1, High Affinity (NTSR1)