NCOA3 ELISA Kit

Catalog No. ABIN2044127

Product Details NCOA3 ELISA Kit

Target: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Minimum Detection Limit: 0.31 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.115 ng/mL

Application Details

Plate: Pre-coated

Protocol: Nuclear Receptor Coactivator 3 (NCOA3) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Receptor Coactivator 3 (NCOA3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Receptor Coactivator 3 (NCOA3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Receptor Coactivator 3 (NCOA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Nuclear Receptor Coactivator 3 (NCOA3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for NCOA3

Target: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Abstract: NCOA3 Products

Synonyms: NCOA3 ELISA Kit, ACTR ELISA Kit, AIB-1 ELISA Kit, AIB1 ELISA Kit, CAGH16 ELISA Kit, CTG26 ELISA Kit, KAT13B ELISA Kit, RAC3 ELISA Kit, SRC-3 ELISA Kit, SRC3 ELISA Kit, TNRC14 ELISA Kit, TNRC16 ELISA Kit, TRAM-1 ELISA Kit, bHLHe42 ELISA Kit, pCIP ELISA Kit, 2010305B15Rik ELISA Kit, AW321064 ELISA Kit, Actr ELISA Kit, Aib1 ELISA Kit, Rac3 ELISA Kit, Src3 ELISA Kit, Tram-1 ELISA Kit, Tram1 ELISA Kit, p/Cip ELISA Kit, pCip ELISA Kit, actr ELISA Kit, aib-1 ELISA Kit, aib1 ELISA Kit, cagh16 ELISA Kit, ctg26 ELISA Kit, kat13b ELISA Kit, pcip ELISA Kit, rac3 ELISA Kit, src-3 ELISA Kit, src3 ELISA Kit, tnrc14 ELISA Kit, tnrc16 ELISA Kit, tram-1 ELISA Kit, xSRC-3 ELISA Kit, nuclear receptor coactivator 3 ELISA Kit, nuclear receptor coactivator 3 S homeolog ELISA Kit, NCOA3 ELISA Kit, Ncoa3 ELISA Kit, ncoa3.S ELISA Kit

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha