OLIG2 ELISA Kit

Catalog No. ABIN2044131

The Human OLIG2 ELISA Kit (ABIN2044131) is a Colorimetric ELISA Kit designed to quantify Human OLIG2.

Quick Overview for OLIG2 ELISA Kit (ABIN2044131)

Target: OLIG2 (Oligodendrocyte Lineage Transcription Factor 2 (OLIG2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62-1000 pg/mL

Application: ELISA

Product Details OLIG2 ELISA Kit

Minimum Detection Limit: 15.62 pg/mL

Sensitivity: < 5.7 pg/mL

Application Details

Plate: Pre-coated

Protocol: Oligodendrocyte Lineage Transcription Factor 2 (OLIG2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Oligodendrocyte Lineage Transcription Factor 2 (OLIG2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Oligodendrocyte Lineage Transcription Factor 2 (OLIG2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Oligodendrocyte Lineage Transcription Factor 2 (OLIG2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Oligodendrocyte Lineage Transcription Factor 2 (OLIG2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for OLIG2

Target: OLIG2 (Oligodendrocyte Lineage Transcription Factor 2 (OLIG2))