PER1 ELISA Kit

Catalog No. ABIN2044195

Product Details PER1 ELISA Kit

Target: PER1 (Period Homolog 1 (Drosophila) (PER1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Minimum Detection Limit: 0.31 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.114 ng/mL

Application Details

Plate: Pre-coated

Protocol: Period Circadian Protein 1 (PER1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Period Circadian Protein 1 (PER1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Period Circadian Protein 1 (PER1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Period Circadian Protein 1 (PER1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Period Circadian Protein 1 (PER1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for PER1

Target: PER1 (Period Homolog 1 (Drosophila) (PER1))

Alternative Name: Period Circadian Protein 1 (PER1) (PER1 Products)

Synonyms: PER ELISA Kit, RIGUI ELISA Kit, hPER ELISA Kit, per1 ELISA Kit, si:ch211-237l4.4 ELISA Kit, Per ELISA Kit, m-rigui ELISA Kit, mPer1 ELISA Kit, rper1 ELISA Kit, Rigui-like ELISA Kit, per4 ELISA Kit, zfper4 ELISA Kit, period circadian regulator 1 ELISA Kit, period circadian clock 1a ELISA Kit, period circadian clock 1 ELISA Kit, period homolog 1 (Drosophila) ELISA Kit, period circadian clock 1b ELISA Kit, PER1 ELISA Kit, per1a ELISA Kit, per1 ELISA Kit, Per1 ELISA Kit, per1b ELISA Kit

Pathways: Photoperiodism