Peroxidasin ELISA Kit
Quick Overview for Peroxidasin ELISA Kit (ABIN2044201)
Target
See all Peroxidasin (PXDN) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 0.15 ng/mL
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Sensitivity
- < 0.059 ng/mL
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Peroxidasin Homolog (PXDN) ELISA Kit
PXDN Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxidasin Homolog (PXDN) ELISA KitPXDN Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxidasin Homolog (PXDN) ELISA KitPXDN Reactivity: Human Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
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Plate
- Pre-coated
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Protocol
- Peroxidasin Homolog (PXDN) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxidasin Homolog (PXDN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxidasin Homolog (PXDN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxidasin Homolog (PXDN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Peroxidasin Homolog (PXDN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- Peroxidasin (PXDN) (Peroxidasin Homolog (PXDN))
Target See all Peroxidasin (PXDN) ELISA Kits
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