PDE1A ELISA Kit

Catalog No. ABIN2044213

Human PDE1A ELISA Kit, Colorimetric assay for quantification of Human PDE1A.

Quick Overview for PDE1A ELISA Kit (ABIN2044213)

Target: PDE1A (Phosphodiesterase 1A (PDE1A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details PDE1A ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.126 ng/mL

Application Details

Plate: Pre-coated

Protocol: Phosphodiesterase 1A, Calmodulin Dependent (PDE1A) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphodiesterase 1A, Calmodulin Dependent (PDE1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphodiesterase 1A, Calmodulin Dependent (PDE1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphodiesterase 1A, Calmodulin Dependent (PDE1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Phosphodiesterase 1A, Calmodulin Dependent (PDE1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for PDE1A

Target: PDE1A (Phosphodiesterase 1A (PDE1A))

Alternative Name: Phosphodiesterase 1A, Calmodulin Dependent (PDE1A)

Pathways: Neurotrophin Signaling Pathway, cAMP Metabolic Process, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma