PLA2G4D ELISA Kit

Catalog No. ABIN2044219

Human PLA2G4D ELISA Kit, Colorimetric assay for quantification of Human PLA2G4D.

Quick Overview for PLA2G4D ELISA Kit (ABIN2044219)

Target: PLA2G4D (phospholipase A2, Group IVD (Cytosolic) (PLA2G4D))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details PLA2G4D ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.064 ng/mL

Application Details

Plate: Pre-coated

Protocol: Phospholipase A2, Group IVD (PLA2G4D) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Group IVD (PLA2G4D). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Group IVD (PLA2G4D). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Group IVD (PLA2G4D), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Phospholipase A2, Group IVD (PLA2G4D) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for PLA2G4D

Target: PLA2G4D (phospholipase A2, Group IVD (Cytosolic) (PLA2G4D))

Alternative Name: Phospholipase A2, Group IVD (PLA2G4D)

Pathways: Inositol Metabolic Process, VEGF Signaling