PIM2 ELISA Kit

Catalog No. ABIN2044227

The Human PIM2 ELISA Kit (ABIN2044227) is a Colorimetric ELISA Kit designed to quantify Human PIM2.

Quick Overview for PIM2 ELISA Kit (ABIN2044227)

Target: PIM2 (Proto-Oncogene Pim-2 (Serine Threonine Kinase) (PIM2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.62-40 ng/mL

Application: ELISA

Product Details PIM2 ELISA Kit

Minimum Detection Limit: 0.62 ng/mL

Sensitivity: < 0.227 ng/mL

Application Details

Plate: Pre-coated

Protocol: Pim-2 Oncogene (PIM2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pim-2 Oncogene (PIM2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pim-2 Oncogene (PIM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pim-2 Oncogene (PIM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Pim-2 Oncogene (PIM2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for PIM2

Target: PIM2 (Proto-Oncogene Pim-2 (Serine Threonine Kinase) (PIM2))

Alternative Name: Pim-2 Oncogene (PIM2)