PIM3 ELISA Kit

Catalog No. ABIN2044229

Product Details PIM3 ELISA Kit

Target: PIM3 (Pim-3 Oncogene (PIM3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.62-40 ng/mL

Minimum Detection Limit: 0.62 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.228 ng/mL

Application Details

Plate: Pre-coated

Protocol: Pim-3 Oncogene (PIM3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pim-3 Oncogene (PIM3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pim-3 Oncogene (PIM3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pim-3 Oncogene (PIM3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Pim-3 Oncogene (PIM3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for PIM3

Target: PIM3 (Pim-3 Oncogene (PIM3))

Abstract: PIM3 Products

Synonyms: pim1-a ELISA Kit, pim-3 ELISA Kit, BC026639 ELISA Kit, Kid1 ELISA Kit, Serine/threonine-protein kinase Pim-3 ELISA Kit, Pim-3 proto-oncogene, serine/threonine kinase S homeolog ELISA Kit, Pim-3 proto-oncogene, serine/threonine kinase ELISA Kit, pim-3 oncogene ELISA Kit, proviral integration site 3 ELISA Kit, pim3 ELISA Kit, pim3.S ELISA Kit, PIM3 ELISA Kit, Pim3 ELISA Kit

Pathways: Negative Regulation of Hormone Secretion