PDGFC ELISA Kit

Catalog No. ABIN2044239

This Rat PDGFC ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat PDGFC.

Quick Overview for PDGFC ELISA Kit (ABIN2044239)

Target: PDGFC (Platelet-Derived Growth Factor C (PDGFC))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details PDGFC ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.113 ng/mL

Application Details

Plate: Pre-coated

Protocol: Platelet Derived Growth Factor C (PDGFC) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor C (PDGFC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet Derived Growth Factor C (PDGFC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Derived Growth Factor C (PDGFC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Platelet Derived Growth Factor C (PDGFC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for PDGFC

Target: PDGFC (Platelet-Derived Growth Factor C (PDGFC))

Alternative Name: Platelet Derived Growth Factor C (PDGFC)

Target Type: Viral Protein

Pathways: RTK Signaling, Platelet-derived growth Factor Receptor Signaling