WBP2NL ELISA Kit

Catalog No. ABIN2044253

This Colorimetric ELISA kit is designed for the quantitative measurement of Cow WBP2NL.

Quick Overview for WBP2NL ELISA Kit (ABIN2044253)

Target: WBP2NL (WBP2 N-terminal Like (WBP2NL))

Reactivity: Cow

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details WBP2NL ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.059 ng/mL

Application Details

Plate: Pre-coated

Protocol: Postacrosomal Sheath WW Domain Binding Protein (PAWP) BioAssay-ELISA Kit (Bovine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Postacrosomal Sheath WW Domain Binding Protein (PAWP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Postacrosomal Sheath WW Domain Binding Protein (PAWP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Postacrosomal Sheath WW Domain Binding Protein (PAWP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Postacrosomal Sheath WW Domain Binding Protein (PAWP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for WBP2NL

Target: WBP2NL (WBP2 N-terminal Like (WBP2NL))

Alternative Name: Postacrosomal Sheath WW Domain Binding Protein (PAWP)