KCNJ5 ELISA Kit

Catalog No. ABIN2044257

This Rat KCNJ5 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat KCNJ5.

Quick Overview for KCNJ5 ELISA Kit (ABIN2044257)

Target: KCNJ5 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 5 (KCNJ5))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details KCNJ5 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.055 ng/mL

Application Details

Plate: Pre-coated

Protocol: Potassium Inwardly Rectifying Channel Subfamily J, Member 5 (KCNJ5) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Potassium Inwardly Rectifying Channel Subfamily J, Member 5 (KCNJ5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Potassium Inwardly Rectifying Channel Subfamily J, Member 5 (KCNJ5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Potassium Inwardly Rectifying Channel Subfamily J, Member 5 (KCNJ5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Potassium Inwardly Rectifying Channel Subfamily J, Member 5 (KCNJ5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for KCNJ5

Target: KCNJ5 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 5 (KCNJ5))

Alternative Name: Potassium Inwardly Rectifying Channel Subfamily J, Member 5 (KCNJ5)

Pathways: Notch Signaling