PA2G4 ELISA Kit

Catalog No. ABIN2044287

The Human PA2G4 ELISA Kit (ABIN2044287) is a Colorimetric ELISA Kit designed to quantify Human PA2G4.

Quick Overview for PA2G4 ELISA Kit (ABIN2044287)

Target: PA2G4 (Proliferation-Associated 2G4, 38kDa (PA2G4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details PA2G4 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.113 ng/mL

Application Details

Plate: Pre-coated

Protocol: Proliferation Associated Protein 2G4 (PA2G4) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proliferation Associated Protein 2G4 (PA2G4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proliferation Associated Protein 2G4 (PA2G4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proliferation Associated Protein 2G4 (PA2G4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Proliferation Associated Protein 2G4 (PA2G4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for PA2G4

Target: PA2G4 (Proliferation-Associated 2G4, 38kDa (PA2G4))

Alternative Name: Proliferation Associated Protein 2G4 (PA2G4)

Pathways: Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Toll-Like Receptors Cascades