F2RL3 ELISA Kit

Catalog No. ABIN2044313

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat F2RL3.

Quick Overview for F2RL3 ELISA Kit (ABIN2044313)

Target: F2RL3 (Coagulation Factor II (Thrombin) Receptor-Like 3 (F2RL3))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details F2RL3 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.059 ng/mL

Application Details

Plate: Pre-coated

Protocol: Protease Activated Receptor 4 (PAR4) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protease Activated Receptor 4 (PAR4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protease Activated Receptor 4 (PAR4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protease Activated Receptor 4 (PAR4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Protease Activated Receptor 4 (PAR4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for F2RL3

Target: F2RL3 (Coagulation Factor II (Thrombin) Receptor-Like 3 (F2RL3))

Alternative Name: Protease Activated Receptor 4 (PAR4)

Pathways: Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process