PIAS3 ELISA Kit

Catalog No. ABIN2044325

This Colorimetric ELISA kit is designed for the quantitative measurement of Human PIAS3.

Quick Overview for PIAS3 ELISA Kit (ABIN2044325)

Target: PIAS3 (Protein Inhibitor of Activated STAT, 3 (PIAS3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details PIAS3 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.064 ng/mL

Application Details

Plate: Pre-coated

Protocol: Protein Inhibitor Of Activated STAT 3 (PIAS3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Inhibitor Of Activated STAT 3 (PIAS3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Inhibitor Of Activated STAT 3 (PIAS3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Inhibitor Of Activated STAT 3 (PIAS3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Protein Inhibitor Of Activated STAT 3 (PIAS3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for PIAS3

Target: PIAS3 (Protein Inhibitor of Activated STAT, 3 (PIAS3))

Alternative Name: Protein Inhibitor Of Activated STAT 3 (PIAS3)

Pathways: JAK-STAT Signaling