PKC delta ELISA Kit

Catalog No. ABIN2044335

This Rat PKC delta ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat PKC delta.

Quick Overview for PKC delta ELISA Kit (ABIN2044335)

Target: PKC delta (PKCd) (Protein Kinase C, delta (PKCd))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details PKC delta ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.053 ng/mL

Application Details

Plate: Pre-coated

Protocol: Protein Kinase C Delta (PKCd) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase C Delta (PKCd). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase C Delta (PKCd). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase C Delta (PKCd), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Protein Kinase C Delta (PKCd) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for PKC delta

Target: PKC delta (PKCd) (Protein Kinase C, delta (PKCd))

Alternative Name: Protein Kinase C delta (PKCd)

Pathways: Interferon-gamma Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, Lipid Metabolism