PCMT1 ELISA Kit

Catalog No. ABIN2044347

The Human PCMT1 ELISA Kit (ABIN2044347) is a Colorimetric ELISA Kit designed to quantify Human PCMT1.

Quick Overview for PCMT1 ELISA Kit (ABIN2044347)

Target: PCMT1 (Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase (PCMT1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25-2000 pg/mL

Application: ELISA

Product Details PCMT1 ELISA Kit

Minimum Detection Limit: 31.25 pg/mL

Sensitivity: < 13.8 pg/mL

Application Details

Plate: Pre-coated

Protocol: Protein L-Isoaspartate-O-Methyltransferase (PCMT1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein L-Isoaspartate-O-Methyltransferase (PCMT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein L-Isoaspartate-O-Methyltransferase (PCMT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein L-Isoaspartate-O-Methyltransferase (PCMT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Protein L-Isoaspartate-O-Methyltransferase (PCMT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for PCMT1

Target: PCMT1 (Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase (PCMT1))

Alternative Name: Protein L-Isoaspartate-O-Methyltransferase (PCMT1)

Pathways: Ribonucleoprotein Complex Subunit Organization