RSPO1 ELISA Kit

Catalog No. ABIN2044371

This Human RSPO1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human RSPO1.

Quick Overview for RSPO1 ELISA Kit (ABIN2044371)

Target: RSPO1 (R-Spondin 1 (RSPO1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62-1000 pg/mL

Application: ELISA

Product Details RSPO1 ELISA Kit

Minimum Detection Limit: 15.62 pg/mL

Sensitivity: < 5.8 pg/mL

Application Details

Plate: Pre-coated

Protocol: R-Spondin 1 (RSPO1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to R-Spondin 1 (RSPO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to R-Spondin 1 (RSPO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain R-Spondin 1 (RSPO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of R-Spondin 1 (RSPO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for RSPO1

Target: RSPO1 (R-Spondin 1 (RSPO1))