RAPSN ELISA Kit

Catalog No. ABIN2044393

Mouse RAPSN ELISA Kit, Colorimetric assay for quantification of Mouse RAPSN.

Quick Overview for RAPSN ELISA Kit (ABIN2044393)

Target: RAPSN (Receptor-Associated Protein of The Synapse (RAPSN))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 ng/mL

Application: ELISA

Product Details RAPSN ELISA Kit

Minimum Detection Limit: 1.56 ng/mL

Sensitivity: < 0.55 ng/mL

Application Details

Plate: Pre-coated

Protocol: Receptor Associated Protein Of The Synapse (RAPSN) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor Associated Protein Of The Synapse (RAPSN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor Associated Protein Of The Synapse (RAPSN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor Associated Protein Of The Synapse (RAPSN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Receptor Associated Protein Of The Synapse (RAPSN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for RAPSN

Target: RAPSN (Receptor-Associated Protein of The Synapse (RAPSN))

Alternative Name: Receptor Associated Protein Of The Synapse (RAPSN)