REG3B ELISA Kit

Catalog No. ABIN2044409

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse REG3B.

Quick Overview for REG3B ELISA Kit (ABIN2044409)

Target: REG3B (Regenerating Islet-Derived 3 beta (REG3B))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 9.37-600 pg/mL

Application: ELISA

Product Details REG3B ELISA Kit

Minimum Detection Limit: 9.37 pg/mL

Sensitivity: < 4.1 pg/mL

Application Details

Plate: Pre-coated

Protocol: Regenerating Islet Derived Protein 3 Beta (REG3b) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regenerating Islet Derived Protein 3 Beta (REG3b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Regenerating Islet Derived Protein 3 Beta (REG3b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regenerating Islet Derived Protein 3 Beta (REG3b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Regenerating Islet Derived Protein 3 Beta (REG3b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for REG3B

Target: REG3B (Regenerating Islet-Derived 3 beta (REG3B))

Alternative Name: Regenerating Islet Derived Protein 3 beta (REG3b)