Rhodopsin ELISA Kit

Catalog No. ABIN2044439

This Colorimetric ELISA kit is designed for the quantitative measurement of Chemical Rhodopsin.

Quick Overview for Rhodopsin ELISA Kit (ABIN2044439)

Target: Rhodopsin (RHO)

Reactivity: Chemical

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 ng/mL

Application: ELISA

Product Details Rhodopsin ELISA Kit

Minimum Detection Limit: 1.56 ng/mL

Brand: BioAssay™

Sensitivity: < 0.64 ng/mL

Application Details

Plate: Pre-coated

Protocol: Rhodopsin (RHO) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rhodopsin (RHO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Rhodopsin (RHO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rhodopsin (RHO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Rhodopsin (RHO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Rhodopsin

Target: Rhodopsin (RHO)

Target Type: Chemical

Pathways: WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction