RBM20 ELISA Kit

Catalog No. ABIN2044477

Human RBM20 ELISA Kit, Colorimetric assay for quantification of Human RBM20.

Quick Overview for RBM20 ELISA Kit (ABIN2044477)

Target: RBM20 (RNA Binding Motif Protein 20 (RBM20))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details RBM20 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.121 ng/mL

Application Details

Plate: Pre-coated

Protocol: RNA Binding Motif Protein 20 (RBM20) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RNA Binding Motif Protein 20 (RBM20). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RNA Binding Motif Protein 20 (RBM20). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RNA Binding Motif Protein 20 (RBM20), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of RNA Binding Motif Protein 20 (RBM20) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for RBM20

Target: RBM20 (RNA Binding Motif Protein 20 (RBM20))