RBM24 ELISA Kit (RNA Binding Motif Protein 24)

Details for Product RBM24 ELISA Kit No. ABIN2044479
Antigen
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
3
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
0.31-20 ng/mL
Minimum Detection Limit
0.31 ng/mL
Application
ELISA
Options
Brand BioAssay™
Detection Method Colorimetric
Sensitivity < 0.123 ng/mL
Antigen
Pathways Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
Plate Pre-coated
Protocol RNA Binding Motif Protein 24 (RBM24) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RNA Binding Motif Protein 24 (RBM24). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RNA Binding Motif Protein 24 (RBM24). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RNA Binding Motif Protein 24 (RBM24), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of RNA Binding Motif Protein 24 (RBM24) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Restrictions For Research Use only
Storage 4 °C/-20 °C