LKB1 ELISA Kit

Catalog No. ABIN2044533

This Mouse LKB1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse LKB1.

Quick Overview for LKB1 ELISA Kit (ABIN2044533)

Target: LKB1 (STK11) (serine/threonine Kinase 11 (STK11))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details LKB1 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.051 ng/mL

Application Details

Plate: Pre-coated

Protocol: Serine/Threonine Kinase 11 (STK11) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine/Threonine Kinase 11 (STK11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serine/Threonine Kinase 11 (STK11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine/Threonine Kinase 11 (STK11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Serine/Threonine Kinase 11 (STK11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for LKB1

Target: LKB1 (STK11) (serine/threonine Kinase 11 (STK11))

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect