SH3BGRL ELISA Kit

Catalog No. ABIN2044543

Human SH3BGRL ELISA Kit, Colorimetric assay for quantification of Human SH3BGRL.

Quick Overview for SH3BGRL ELISA Kit (ABIN2044543)

Target: SH3BGRL (SH3 Domain Binding Glutamic Acid-Rich Protein Like (SH3BGRL))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details SH3BGRL ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.061 ng/mL

Application Details

Plate: Pre-coated

Protocol: SH3 Domain Binding Glutamic Acid Rich Protein Like Protein (SH3BGRL) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SH3 Domain Binding Glutamic Acid Rich Protein Like Protein (SH3BGRL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SH3 Domain Binding Glutamic Acid Rich Protein Like Protein (SH3BGRL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SH3 Domain Binding Glutamic Acid Rich Protein Like Protein (SH3BGRL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of SH3 Domain Binding Glutamic Acid Rich Protein Like Protein (SH3BGRL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for SH3BGRL

Target: SH3BGRL (SH3 Domain Binding Glutamic Acid-Rich Protein Like (SH3BGRL))

Alternative Name: SH3 Domain Binding Glutamic Acid Rich Protein Like Protein (SH3BGRL)