SPON2 ELISA Kit

Catalog No. ABIN2044603

Product Details SPON2 ELISA Kit

Target: SPON2 (Spondin 2 (SPON2))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.062 ng/mL

Application Details

Plate: Pre-coated

Protocol: Spondin 2 (SPON2) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Spondin 2 (SPON2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Spondin 2 (SPON2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Spondin 2 (SPON2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Spondin 2 (SPON2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for SPON2

Target: SPON2 (Spondin 2 (SPON2))

Abstract: SPON2 Products

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