KIT Ligand ELISA Kit

Catalog No. ABIN2044605

This Cow KIT Ligand ELISA Kit is a Colorimetric ELISA Kit designed to quantify Cow KIT Ligand.

Quick Overview for KIT Ligand ELISA Kit (ABIN2044605)

Target: KIT Ligand (KITLG)

Reactivity: Cow

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62-1000 pg/mL

Application: ELISA

Product Details KIT Ligand ELISA Kit

Minimum Detection Limit: 15.62 pg/mL

Sensitivity: < 5.7 pg/mL

Application Details

Plate: Pre-coated

Protocol: Stem Cell Factor (SCF) BioAssay-ELISA Kit (Bovine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stem Cell Factor (SCF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Stem Cell Factor (SCF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Stem Cell Factor (SCF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Stem Cell Factor (SCF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for KIT Ligand

Target: KIT Ligand (KITLG)

Alternative Name: Stem Cell Factor (SCF)

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway