Superoxide Dismutases ELISA Kit
Quick Overview for Superoxide Dismutases ELISA Kit (ABIN2044629)
Target
Reactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 0.15 ng/mL
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Sensitivity
- < 0.063 ng/mL
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Superoxide Dismutases ELISA Kit
Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Superoxide Dismutases ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Superoxide Dismutases ELISA KitReactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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Plate
- Pre-coated
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Protocol
- Superoxide Dismutases (SOD) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Superoxide Dismutases (SOD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Superoxide Dismutases (SOD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Superoxide Dismutases (SOD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Superoxide Dismutases (SOD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- Superoxide Dismutases
Target
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