T-Bet ELISA Kit
Quick Overview for T-Bet ELISA Kit (ABIN2044645)
Target
See all T-Bet ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 0.15 ng/mL
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Sensitivity
- < 0.052 ng/mL
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T-Bet ELISA Kit
Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate
T-Bet ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
T-Bet ELISA KitReactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
T-Bet ELISA KitReactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate
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Plate
- Pre-coated
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Protocol
- T-Box Protein 21 (TBX21) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T-Box Protein 21 (TBX21). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to T-Box Protein 21 (TBX21). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain T-Box Protein 21 (TBX21), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of T-Box Protein 21 (TBX21) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- T-Bet
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Alternative Name
- T-Box Protein 21 (TBX21)
Target See all T-Bet ELISA Kits
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