TJP1 ELISA Kit

Catalog No. ABIN2044667

Product Details TJP1 ELISA Kit

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.051 ng/mL

Application Details

Plate: Pre-coated

Protocol: Tight Junction Protein 1 (TJP1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tight Junction Protein 1 (TJP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tight Junction Protein 1 (TJP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tight Junction Protein 1 (TJP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Tight Junction Protein 1 (TJP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for TJP1

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Abstract: TJP1 Products

Synonyms: ZO-1 ELISA Kit, ZO1 ELISA Kit, zo1 ELISA Kit, tight junction protein 1 ELISA Kit, tight junction protein ZO-1 ELISA Kit, TJP1 ELISA Kit, Tjp1 ELISA Kit, LOC396567 ELISA Kit

Pathways: Carbohydrate Homeostasis, Cell-Cell Junction Organization