TICAM1 ELISA Kit

Catalog No. ABIN2044679

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse TICAM1.

Quick Overview for TICAM1 ELISA Kit (ABIN2044679)

Target: TICAM1 (Toll-Like Receptor Adaptor Molecule 1 (TICAM1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Application: ELISA

Product Details TICAM1 ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Sensitivity: < 22.9 pg/mL

Application Details

Plate: Pre-coated

Protocol: Toll Like Receptor Adaptor Molecule 1 (TICAM1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Toll Like Receptor Adaptor Molecule 1 (TICAM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Toll Like Receptor Adaptor Molecule 1 (TICAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Toll Like Receptor Adaptor Molecule 1 (TICAM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Toll Like Receptor Adaptor Molecule 1 (TICAM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for TICAM1

Target: TICAM1 (Toll-Like Receptor Adaptor Molecule 1 (TICAM1))

Alternative Name: Toll Like Receptor Adaptor Molecule 1 (TICAM1)

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades