Tppp ELISA Kit

Catalog No. ABIN2044717

Human Tppp ELISA Kit, Colorimetric assay for quantification of Human Tppp.

Quick Overview for Tppp ELISA Kit (ABIN2044717)

Target: Tppp (Tubulin Polymerization Promoting Protein (Tppp))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details Tppp ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.132 ng/mL

Application Details

Plate: Pre-coated

Protocol: Tubulin Polymerization Promoting Protein (TPPP) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tubulin Polymerization Promoting Protein (TPPP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tubulin Polymerization Promoting Protein (TPPP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tubulin Polymerization Promoting Protein (TPPP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Tubulin Polymerization Promoting Protein (TPPP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Tppp

Target: Tppp (Tubulin Polymerization Promoting Protein (Tppp))