USP14 ELISA Kit

Catalog No. ABIN2044745

Product Details USP14 ELISA Kit

Target: USP14 (Ubiquitin Specific Peptidase 14 (USP14))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Minimum Detection Limit: 0.31 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.108 ng/mL

Application Details

Plate: Pre-coated

Protocol: Ubiquitin Specific Peptidase 14 (USP14) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Specific Peptidase 14 (USP14). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin Specific Peptidase 14 (USP14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin Specific Peptidase 14 (USP14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ubiquitin Specific Peptidase 14 (USP14) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for USP14

Target: USP14 (Ubiquitin Specific Peptidase 14 (USP14))

Abstract: USP14 Products

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