ERBB3 ELISA Kit

Catalog No. ABIN2044771

Human ERBB3 ELISA Kit Colorimetric assay for quantification of Human ERBB3.

Quick Overview for ERBB3 ELISA Kit (ABIN2044771)

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.12-5000 pg/mL

Application: ELISA

Product Details ERBB3 ELISA Kit

Minimum Detection Limit: 78.12 pg/mL

Sensitivity: < 32 pg/mL

Application Details

Plate: Pre-coated

Protocol: V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for ERBB3

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Alternative Name: V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3)

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway