RSAD2 ELISA Kit
Quick Overview for RSAD2 ELISA Kit (ABIN2044793)
Target
See all RSAD2 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 0.15 ng/mL
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Sensitivity
- < 0.062 ng/mL
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Plate
- Pre-coated
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Protocol
- Viperin BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Viperin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Viperin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Viperin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Viperin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- RSAD2 (Radical S-Adenosyl Methionine Domain Containing 2 (RSAD2))
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Alternative Name
- Viperin
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Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
Target See all RSAD2 ELISA Kits
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