WFDC2 ELISA Kit

Catalog No. ABIN2044803

This Human WFDC2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human WFDC2.

Quick Overview for WFDC2 ELISA Kit (ABIN2044803)

Target: WFDC2 (WAP Four-Disulfide Core Domain 2 (WFDC2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details WFDC2 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Brand: BioAssay™

Sensitivity: < 0.114 ng/mL

Application Details

Plate: Pre-coated

Protocol: WAP Four Disulfide Core Domain Protein 2 (WFDC2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to WAP Four Disulfide Core Domain Protein 2 (WFDC2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WAP Four Disulfide Core Domain Protein 2 (WFDC2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WAP Four Disulfide Core Domain Protein 2 (WFDC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of WAP Four Disulfide Core Domain Protein 2 (WFDC2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for WFDC2

Target: WFDC2 (WAP Four-Disulfide Core Domain 2 (WFDC2))

Alternative Name: WAP Four Disulfide Core Domain Protein 2 (WFDC2)