WFDC2 ELISA Kit

Catalog No. ABIN5510648

The Human WFDC2 ELISA Kit (ABIN5510648) is a Colorimetric ELISA Kit designed to quantify Human WFDC2. This ELISA Kit has been cited in 1 publication.

Quick Overview for WFDC2 ELISA Kit (ABIN5510648)

Target: WFDC2 (WAP Four-Disulfide Core Domain 2 (WFDC2))

Binding Specificity: AA 31-124

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine

Product Details WFDC2 ELISA Kit

Minimum Detection Limit: 156 pg/mL

Purpose: Sandwich Human HE4 ELISA Kit to quantitate Human WFDC2 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: E31-F124

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from sheep

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 15 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 5.7%, 6.8%, 4%
Inter-Assay Precision (CV%): 6.4%, 8.6%, 4.3%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for WFDC2 ELISA Kit (ABIN5510648)

Dong, Liu, Li: "Value of HE4 Combined with Cancer Antigen 125 in the Diagnosis of Endometrial Cancer." in: Pakistan journal of medical sciences, Vol. 33, Issue 4, pp. 1013-1017, (2017) (PubMed).

Target Details for WFDC2

Target: WFDC2 (WAP Four-Disulfide Core Domain 2 (WFDC2))

Alternative Name: WFDC2

Background:

Background: WAP four-disulfide core domain protein 2, also known as Human Epididymis Protein 4 (HE4), is a protein that in humans is encoded by the WFDC2 gene. This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation.

Gene Full Name: WAP four-disulfide core domain 2

Gene ID: 10406

UniProt: Q14508