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WFDC2 ELISA Kit

WFDC2 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Catalog No. ABIN5690889
  • Target See all WFDC2 ELISA Kits
    WFDC2 (WAP Four-Disulfide Core Domain 2 (WFDC2))
    Reactivity
    • 5
    • 2
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human HE4/WFDC2 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes Human HE4.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    6 months
  • Zhou, Lv, Shao, Xu, Luo, Zhang, Hu, Zhang, Luo, Fang, Wang, Duan, Huang: "Detection of Human Epididymis Protein 4 (HE4) in Human Serum Samples Using a Specific Monoclonal Antibody-Based Sandwich Enzyme-Linked Immunosorbent Assay (ELISA)." in: Journal of clinical laboratory analysis, Vol. 30, Issue 5, pp. 581-9, (2017) (PubMed).

  • Target See all WFDC2 ELISA Kits
    WFDC2 (WAP Four-Disulfide Core Domain 2 (WFDC2))
    Alternative Name
    HE4 (WFDC2 (WFDC2 Products)
    Synonyms
    WFDC3 ELISA Kit, WFDC2 ELISA Kit, si:ch211-153a8.3 ELISA Kit, EDDM4 ELISA Kit, HE4 ELISA Kit, WAP5 ELISA Kit, dJ461P17.6 ELISA Kit, 1600023A02Rik ELISA Kit, re4 ELISA Kit, CE4 ELISA Kit, BE-20 ELISA Kit, WAP four-disulfide core domain 2 ELISA Kit, WFDC2 ELISA Kit, wfdc2 ELISA Kit, Wfdc2 ELISA Kit
    Gene ID
    10406
    UniProt
    Q14508
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