WASF2 ELISA Kit

Catalog No. ABIN2044845

This Mouse WASF2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse WASF2.

Quick Overview for WASF2 ELISA Kit (ABIN2044845)

Target: WASF2 (WAS Protein Family, Member 2 (WASF2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details WASF2 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.053 ng/mL

Application Details

Plate: Pre-coated

Protocol: Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for WASF2

Target: WASF2 (WAS Protein Family, Member 2 (WASF2))

Alternative Name: Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2)

Pathways: RTK Signaling