XBP1 ELISA Kit

Catalog No. ABIN2044857

Product Details XBP1 ELISA Kit

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.054 ng/mL

Application Details

Plate: Pre-coated

Protocol: X-Box Binding Protein 1 (XBP1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to X-Box Binding Protein 1 (XBP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to X-Box Binding Protein 1 (XBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain X-Box Binding Protein 1 (XBP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of X-Box Binding Protein 1 (XBP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for XBP1

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Abstract: XBP1 Products

Synonyms: treb5 ELISA Kit, xXBP1 ELISA Kit, xbp2 ELISA Kit, XBP1P1 ELISA Kit, XBPP1 ELISA Kit, xbp1 ELISA Kit, TREB5 ELISA Kit, XBP-1 ELISA Kit, XBP2 ELISA Kit, D11Ertd39e ELISA Kit, TREB-5 ELISA Kit, HTF ELISA Kit, cb918 ELISA Kit, id:ibd1195 ELISA Kit, trebf ELISA Kit, wu:fb02h05 ELISA Kit, wu:fb63f10 ELISA Kit, xbp1a ELISA Kit, xbp1b ELISA Kit, X-box binding protein 1 ELISA Kit, X-box binding protein 1 L homeolog ELISA Kit, xbp1 ELISA Kit, XBP1 ELISA Kit, Xbp1 ELISA Kit, xbp1.L ELISA Kit

Pathways: ER-Nucleus Signaling, Unfolded Protein Response