XBP1 ELISA Kit

Catalog No. ABIN2044857

The Rat XBP1 ELISA Kit (ABIN2044857) is a Colorimetric ELISA Kit designed to quantify Rat XBP1.

Quick Overview for XBP1 ELISA Kit (ABIN2044857)

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details XBP1 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.054 ng/mL

Application Details

Plate: Pre-coated

Protocol: X-Box Binding Protein 1 (XBP1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to X-Box Binding Protein 1 (XBP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to X-Box Binding Protein 1 (XBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain X-Box Binding Protein 1 (XBP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of X-Box Binding Protein 1 (XBP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for XBP1

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Pathways: ER-Nucleus Signaling, Unfolded Protein Response