YAP1 ELISA Kit

Catalog No. ABIN2044861

Mouse YAP1 ELISA Kit, Colorimetric assay for quantification of Mouse YAP1.

Quick Overview for YAP1 ELISA Kit (ABIN2044861)

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details YAP1 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.056 ng/mL

Application Details

Plate: Pre-coated

Protocol: Yes Associated Protein 1 (YAP1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Yes Associated Protein 1 (YAP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Yes Associated Protein 1 (YAP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Yes Associated Protein 1 (YAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Yes Associated Protein 1 (YAP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for YAP1

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Alternative Name: Yes Associated Protein 1 (YAP1)

Pathways: MAPK Signaling, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha