ZP2 ELISA Kit

Catalog No. ABIN2044869

The Rat ZP2 ELISA Kit (ABIN2044869) is a Colorimetric ELISA Kit designed to quantify Rat ZP2.

Quick Overview for ZP2 ELISA Kit (ABIN2044869)

Target: ZP2 (Zona Pellucida Glycoprotein 2 (ZP2))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details ZP2 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.125 ng/mL

Application Details

Plate: Pre-coated

Protocol: Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for ZP2

Target: ZP2 (Zona Pellucida Glycoprotein 2 (ZP2))

Alternative Name: Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2)