Hepcidin ELISA Kit
Quick Overview for Hepcidin ELISA Kit (ABIN2111986)
Target
See all Hepcidin (HAMP) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 3.7 ng/mL
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Analytical Method
- Quantitative
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Sensitivity
- < 1.48 ng/mL
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Components
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A: Microtiter Plate, 96 wells, Pre-coated, ready to use
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A, 1x120 µL
E: Detection Reagent B, 1x120 µL
F: Assay Diluent A, 1x10 mL
G: Assay Diluent B, 1x10 mL
H: TMB Substrate, 1x0 mL
K: Stop Solution, 1x0 mL
L: Wash Buffer, 30X, 1x20 mL
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Hepcidin Antimicrobial Peptide (HAMP) ELISA Kit
HAMP Reactivity: Human Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Hepcidin Antimicrobial Peptide (HAMP) ELISA KitHAMP Reactivity: Human Colorimetric Competition ELISA 320 pg/mL - 200000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Hepcidin Antimicrobial Peptide (HAMP) ELISA KitHAMP Reactivity: Mouse Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Urine
Hepcidin Antimicrobial Peptide (HAMP) ELISA KitHAMP Reactivity: Mouse Colorimetric Competition ELISA 1.23 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Hepcidin Antimicrobial Peptide (HAMP) ELISA KitHAMP Reactivity: Rat Colorimetric Competition ELISA 160 pg/mL - 100000 pg/mL Biological Fluids, Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Hepcidin Antimicrobial Peptide (HAMP) ELISA KitHAMP Reactivity: Pig Colorimetric Competition ELISA 740.74 pg/mL - 60000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Hepcidin Antimicrobial Peptide (HAMP) ELISA KitHAMP Reactivity: Mouse Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma, Serum
Hepcidin Antimicrobial Peptide (HAMP) ELISA KitHAMP Reactivity: Horse Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Plasma, Serum, Tissue Homogenate
Hepcidin Antimicrobial Peptide (HAMP) ELISA KitHAMP Reactivity: Mouse Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Hepcidin Antimicrobial Peptide (HAMP) ELISA KitHAMP Reactivity: Human Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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Plate
- Pre-coated
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Protocol
- The Porcine Hepcidin (Hepc) ELISA Kit is a competitive inhibition immunoassay for the in vitro quantitative measurement of Hepc in porcine serum, plasma, tissue homogenates, cell lysates, culture supernatants and other biological fluids.This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for Hepc has been pre-coated onto a microplate. A competitive inhibition reaction occurs between biotin-labeled Hepc and unlabeled Hepc (standards or samples) for limited binding sites on the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is inversely proportional to the concentration of Hepc in the sample. After addition of the substrate solution, the intensity of color developed is inversely proportional to the concentration of Hepc in the sample.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- Hepcidin (HAMP) (Hepcidin Antimicrobial Peptide (HAMP))
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Alternative Name
- Hepcidin (Hepc)
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Pathways
- Hormone Activity, Transition Metal Ion Homeostasis
Target See all Hepcidin (HAMP) ELISA Kits
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