Hepcidin ELISA Kit

Catalog No. ABIN2111986

Product Details Hepcidin ELISA Kit

Target: Hepcidin (HAMP) (Hepcidin Antimicrobial Peptide (HAMP))

Reactivity: Pig

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 3.7-300 ng/mL

Minimum Detection Limit: 3.7 ng/mL

Application: ELISA

Brand: BioAssay™

Analytical Method: Quantitative

Sensitivity: < 1.48 ng/mL

Components: A: Microtiter Plate, 96 wells, Pre-coated, ready to use
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A, 1x120 µL
E: Detection Reagent B, 1x120 µL
F: Assay Diluent A, 1x10 mL
G: Assay Diluent B, 1x10 mL
H: TMB Substrate, 1x0 mL
K: Stop Solution, 1x0 mL
L: Wash Buffer, 30X, 1x20 mL

Application Details

Plate: Pre-coated

Protocol: The Porcine Hepcidin (Hepc) ELISA Kit is a competitive inhibition immunoassay for the in vitro quantitative measurement of Hepc in porcine serum, plasma, tissue homogenates, cell lysates, culture supernatants and other biological fluids.This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for Hepc has been pre-coated onto a microplate. A competitive inhibition reaction occurs between biotin-labeled Hepc and unlabeled Hepc (standards or samples) for limited binding sites on the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is inversely proportional to the concentration of Hepc in the sample. After addition of the substrate solution, the intensity of color developed is inversely proportional to the concentration of Hepc in the sample.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Hepcidin

Target: Hepcidin (HAMP) (Hepcidin Antimicrobial Peptide (HAMP))

Alternative Name: Hepcidin (Hepc) (HAMP Products)

Synonyms: HEPC ELISA Kit, HFE2B ELISA Kit, LEAP1 ELISA Kit, PLTR ELISA Kit, Hepcidin ELISA Kit, Hamp1 ELISA Kit, Hepc ELISA Kit, Hepc1 ELISA Kit, HAMP ELISA Kit, hepcidin ELISA Kit, hamp1 ELISA Kit, hep1 ELISA Kit, hepcidin antimicrobial peptide ELISA Kit, hepcidin-like ELISA Kit, hepcidin-1 ELISA Kit, Ham percentage ELISA Kit, HAMP ELISA Kit, Hamp ELISA Kit, hamp ELISA Kit, LOC100135935 ELISA Kit, LOC106584587 ELISA Kit

Pathways: Hormone Activity, Transition Metal Ion Homeostasis