GM-CSF ELISA Kit

Catalog No. ABIN2111989

Mouse GM-CSF ELISA Kit Colorimetric assay for quantification of Mouse GM-CSF.

Quick Overview for GM-CSF ELISA Kit (ABIN2111989)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 50-3200 pg/mL

Application: ELISA

Product Details GM-CSF ELISA Kit

Minimum Detection Limit: 50 pg/mL

Analytical Method: Quantitative

Sensitivity: < 17 pg/mL

Components: MOUSE GM-CSF MICROTITER PLATE
BIOTIN CONJUGATE
AVIDIN CONJUGATE
MOUSE GM-CSF STANDARD
CALIBRATOR DILUENT I
CALIBRATOR DILUENT II
WASH BUFFER (20X/96 wells, 30X/192 wells)
SUBSTRATE A
SUBSTRATE B
STOP SOLUTION

Application Details

Plate: Pre-coated

Protocol: This ELISA kit provides a tool for studying GM-CSF expression and regulation in animal model.This mouse GM-CSF enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for mouse GM-CSF. Standards or samples are then added to the appropriate microtiter plate wells and incubated. Mouse GM-CSF, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound mouse GM- CSF and other components of the sample. In order to quantitatively determine the amount of mouse GM-CSF present in the sample, a standardized preparation of horseradish peroxidase HRP-conjugated monoclonal antibody specific for mouse GM-CSF is added to each well to -sandwich- the mouse GM-CSF immobilized during the first incubation. The microtiter plate then undergoes a second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3-5,5- tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain mouse GM-CSF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2nm.

Restrictions: For Research Use only

Handling

Target Details for GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: Granulocyte Macrophage Colony Stimulating Factor

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin