Interferon gamma ELISA Kit

Catalog No. ABIN2111992

Mouse Interferon gamma ELISA Kit, Colorimetric assay for quantification of Mouse Interferon gamma.

Quick Overview for Interferon gamma ELISA Kit (ABIN2111992)

Target: Interferon gamma (IFNG)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 50-3200 pg/mL

Application: ELISA

Product Details Interferon gamma ELISA Kit

Minimum Detection Limit: 50 pg/mL

Sensitivity: < 7.5 pg/mL

Components: MOUSE IFn-y MICROTITER PLATE
MOUSE IFn-y BIOTIN CONJUGATE
AVIDIN HRP CONJUGATE
MOUSE IFn-y STANDARD
CALIBRATOR DILUENT I
CALIBRATOR DILUENT II
WASH BUFFER (20X/96 wells, 30X/192 wells)
SUBSTRATE A
SUBSTRATE B
STOP SOLUTION

Application Details

Plate: Pre-coated

Protocol: This mouse IFN-y ELISA is a 4.5 hour solid phase immunoassay readily applicable to measure IFN-y levels in mouse serum, cell culture supernatant, and other biological fluids in the range of 0 to 3200pg/ml. It is expected to be useful for investigations into the relationship between IFN-y and various diseases.This mouse IFN-y enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre- coated with a monoclonal specific for mouse IFN-u. Standards or samples are then added to the appropriate microtiter plate wells and incubated. In order to quantitatively determine the amount of mouse IFN-u present in the sample, a biotin-conjugated antibody specific for mouse IFN-y is added to each well to "sandwich" the IFN-u immobilized during the first incubation. The microtiter plate then undergoes a second incubation and the wells are thoroughly washed to remove all unbound Biotin-conjugated antibodies. Avidin can bind to biotin with high affinity and amplify the biotin signal. A preparation of avidin conjugated HRP is added to each well and incubated. The avidin HRP conjugate will bind to wells containing biotin. After washing the wells, the unbounded avidin-HRP conjugate is removed. A TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IFN-y and biotin-conjugated antibody, avidin conjugated HRP will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Restrictions: For Research Use only

Handling

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy