IL-2 ELISA Kit

Catalog No. ABIN2111997

This Mouse IL-2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse IL-2.

Quick Overview for IL-2 ELISA Kit (ABIN2111997)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.8-500 pg/mL

Application: ELISA

Product Details IL-2 ELISA Kit

Minimum Detection Limit: 7.8 pg/mL

Analytical Method: Quantitative

Sensitivity: < 2.1 pg/mL

Components: MOUSE IL-2 MICROTITER PLATE
BIOTIN CONJUGATE
AVIDIN CONJUGATE
MOUSE IL-2 STANDARD
CALIBRATOR DILUENT I
CALIBRATOR DILUENT II
WASH BUFFER (20X/96 wells, 30X/192 wells)
TMB SUBSTRATE
STOP SOLUTION

Application Details

Plate: Pre-coated

Protocol: This mouse IL-2 enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for mouse IL-2. Standards or samples are then added to the appropriate microtiter plate wells and incubated. Mouse IL-2, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound mouse IL-2 and other components in the samples. A biotin-conjugated antibody preparation specific for mouse IL-2 is added to each well and incubated. The biotin labelled antibody will attach to the wells by binding to mouse IL-2 present in the standards/samples. After plate washing, other proteins, components and unattached biotin labelled antibody are removed. Then, avidin-horseradish peroxidase (HRP) conjugate is added to each well. Avidin has a very high affinity for biotin, thus, it links the tracer (HRP) sturdily to the biotin conjugated antibody. The wells are thoroughly washed to remove all unbound avidin-HRPconjugate and aTMB(3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only the wells that contain mouse IL-2 will exhibit a change in color. The extent of color change is proportional to the quantity of mouse IL-2 present in the standards/samples. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: Interleukin-2

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation