IL-7 ELISA Kit

Catalog No. ABIN2112003

This Human IL-7 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human IL-7.

Quick Overview for IL-7 ELISA Kit (ABIN2112003)

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.5-500 pg/mL

Application: ELISA

Product Details IL-7 ELISA Kit

Minimum Detection Limit: 1.5 pg/mL

Sensitivity: < 1 pg/mL

Components: IL-7 MICROTITER PLATE
BIOTIN CONJUGATE
AVIDIN CONJUGATE
IL-7 STANDARD
CALIBRATOR DILUENT I
CALIBRATOR DILUENT II
WASH BUFFER (20X/96 wells, 30X/192 wells)
SUBSTRATE A
SUBSTRATE B
STOP SOLUTION

Application Details

Plate: Pre-coated

Protocol: This IL-7 ELISA is a ready-to-use 3.5-hour solid phase immunoassay readily capable of measuring IL-7 levels in serum, plasma, cell culture supernatant, and other biological fluids in the range of 0 to 2000pg/ml. This assay has shown no cross-reactivity with other cytokines tested, and is expected to be used effectively for further investigations into the relationship between IL-7 and the various conditions mentioned.This IL-7 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IL-7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-7 and incubated. If present, IL-7 will bind and become immobilized by the antibody pre-coated on the wells and then become -sandwiched- by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound IL- 7 and other components of the sample. In order to quantitatively determine the amount of IL-7 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits, each having a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5,5' tetramethyl- benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-7, biotin- conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for IL-7

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Alternative Name: Interleukin-7

Pathways: JAK-STAT Signaling